ALK1 (ACVRL1) Kinase Assay Kit

Catalog #

79549

$535 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The ALK1 Kinase Assay Kit is designed to measure ALK1 kinase activity for screening and profiling applications using ADP-Glo^® Kinase Assay as a detection reagent. The ALK1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant ALK1 enzyme, ALK1 substrate, ATP and kinase assay buffer for 100 enzyme reactions.

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Synonyms

activin receptor-like kinase 1, ACVRL1

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Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The ALK1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant ALK1 enzyme, ALK1 substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

Format

Catalog #	Reagent	Amount	Storage
40018	ALK1	10 µg	-80°C	*Avoid* *multiple* *freeze/* *thaw* *cycles!*
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 µM)	50 µl	-20°C
	Casein (5 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

P37023

Background

ALK1, also known as activin receptor-like kinase 1 (ACVRL1) is a type I receptor from the TGF-β family of transmembrane serine/threonine kinases. Mutation of ALK1 is seen to play a role in pulmonary arterial hypertension (PAH), Osler-Weber-Rendu syndrome, and vascular smooth muscle cell development.

References

Cunha, Sara I., and Kristian Pietras. “ALK1 as an Emerging Target for Antiangiogenic Therapy of Cancer.” Blood 117(26): 6999–7006 (2011).