ALK1, GST-tag

Catalog #: 40018
Size: 10 µg
$370
Species: Human
Host Species: Sf9 insect cells
MW: 64 kDa
Genbank #: NM_000020
Tag(s): N-terminal GST-tag
a.a: 144–end
Background:
ALK1 is a serine/threonine-protein kinase receptor R3 precursor that mediates signal by TGFβ superfamily (1). ALK1 functions as a TGFβ type I receptor in endothelial cells and is responsible for human hereditary hemorrhagic telangiectasia (HHT) type II. Distinct Smad proteins (i.e., Smad2/Smad3 and Smad1/Smad5) show interaction with ALK1 and mediate TGFβ signaling. Northern blot and RT-PCR analysis show that ALK1 specifically induces expression of Smad6, Smad7, Id1, Id2, endoglin, STAT1, and interleukin 1 receptor in endothelial cells. ALK1 expression in inflammatory myofibroblastic tumor of the urinary bladder have also has been reported (2).
Description:
Recombinant human activin receptor-like kinase 1, or ALK1, (a.a. 144-end), with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. The gene accession number is NM_000020.
Synonym(s):
ACVRL1, ACVRLK1, ALK1, HHT, HHT2, ORW2, SKR3, ALK1, TSR-I
Purity:
≥70%
Specific Activity:
13 pmol/min/µg
Assay Conditions:
Assay was done in Kinase buffer containing 1 mM DTT using Casein substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. The amount of ATP transferred from autophosphorylation as well as substrate phosphorylation was calculated using KinaseGlo reagent (Promega).
Formulation:
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 0.2mM EDTA, 3mM DTT and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Ota, T. et al . J. Cell Physiol. 2002;193(3):299-318.
2. Tsuzuki, T. et al. Am. J. Surg. Pathol. 2004; 28(12):1609-14.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
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Product Datasheets

40018_Lot_180430-A.pdf