ALK , GST-tag

Catalog #: 40214
Size: 10 µg
$345
Species: Human
Host Species: Sf9 insect cells
MW: 89 kDa
Genbank #: NM_004304
Tag(s): N-terminal GST-tag
a.a: 1058–1620 (end)
Description:
Human anaplastic lymphoma receptor tyrosine kinase (ALK), also known as CD246 and NBLST3, (GenBank Accession No. NM_004304), a.a. 1058-1620 (end) with N-terminal GST-tag, MW = 89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q9UM73
Synonym(s):
ALK, CD246, anaplastic lymphoma receptor tyrosine kinase, NBLST3
Specific Activity:
77 pmol/min/µg.
Assay Conditions:
Assay was done in a Kinase buffer using 0.2 mg/ml IGF1Rtide as a substrate with 20 µM ATP at 30°C for 40 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Ren, H., et al., Cancer Res. 2012,72(13):3312-3323.
2. Zhu, S., et al., Cancer Cell 2012,21(3):362-373.
3. Schonherr, C., et al., Crit Rev Oncog. 2012,17(2):123-143
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Receptor Tyrosine
Product Type:
Recombinant Protein
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Product Datasheets

40214_Lot120919-GC2.pdf