Human acidic Fibroblast Growth Factor Recombinant

Catalog #

90101-A

$130 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human aFGF (acidic Fibroblast Growth Factor) is a disulfide-linked monomer protein consisting of 141 a.a. and migrates as an approximately 16 kDa protein under reducing and non-reducing conditions. Native human aFGF, generated by the proteolytic removal of the signal peptide and propeptide, has a calculated mass of 16 kDa. Optimized DNA sequence encoding human acidic Fibroblast Growth Factor mature chain was expressed in E. coli.

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Synonyms

aFGF, acidic Fibroblast Growth Factor, Heparin-Binding Growth Factor 1, HBGF-1, FGF-1, Fibroblast Growth Factor-1

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥98% by SDS-PAGE and HPLC

Format

lyophilized protein

Formulation

Lyophilized from 0.2 µm filtered solution containing 2.5% glycine, 0.5% sucrose, 0.01% Tween-80, 5 mM glutamic acid, pH 4.5.

16 kDa

Endotoxin Level

<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

Amino Acids

16–155

Biological Activity

The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 10 ng/ml, corresponding to a specific activity of ≥1 x 10⁵ units/mg.

Genbank #

P05230

UniProt #

P05230

Background

aFGF and bFGF display 55% homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL-1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation.

References

1. Expression of acidic fibroblast growth factor (aFGF) and fibroblast growth factor receptor 4 (fgfr4) in breast fibroadenomas. J. Clin. Pathol., Jan 2001, 54: 37 -41.
2. Requirement of phosphatidylinositol 3-kinase activity for translocation of exogenous aFGF to the cytosol and nucleus. J. Biol. Chem., Apr 2000, 275: 11972.

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Human acidic Fibroblast Growth Factor Recombinant

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