ADCC Bioassay Effector Cell F Variant (Low Affinity)/ NFAT Luciferase Reporter Jurkat Cell Line

Catalog #
60540
$6,250 *
Size: 2 vials
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

ADCC Bioassay Effector Cell F Variant (Low Affinity)/ NFAT Luciferase Reporter Jurkat Cell Line is an engineered Jurkat T cell line expressing firefly luciferase under the control of NFAT response elements, and human FcγRIIIa low affinity (F158) variant and Fcγ chain. This cell line was functionally validated by ADCC (antibody-dependent cell-mediated cytotoxicity) assays. 

Figure 1: Illustration of the mechanism of action of ADCC Bioassay Effector Cell F Variant (Low Affinity)/NFAT Luciferase Reporter Jurkat Cell Line.
ADCC Bioassay Effector Cell F Variant (Low Affinity)/NFAT Luciferase Reporter Jurkat cells are used as effector cells. The effector cells are co-cultured in the presence of target cells and an antibody of interest. The antibody binds to the target antigen on the target cell whereas its Fc portion binds to FcγRIIIa on the cell surface of the effector cell, cross-linking the effector and target cells. Engagement of FcγRIIIa leads to the activation of the NFAT pathway in the effector cells. Luciferase activity is proportional to the activation of the ADCC cascade.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of FcγRIIIa low affinity (F158) variant without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
Antibody-dependent cell-mediated cytotoxicity, Fc gamma receptor, Fcgamma receptor
Product Info
Storage and Usage
Citations
Host Cell Line
Jurkat is a human leukemia cell line, Non-adherent T lymphocytes.
Supplied As
Each vial contains >1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Materials Required But Not Supplied

Media Required for Cell Culture

Name Ordering Information
Thaw Medium 2 BPS Bioscience #60184
Growth Medium 2A BPS Bioscience #60190

 

Materials Required for Cellular Assays

Name Ordering Information
Anti-hHER2 human IgG1 Antibody R&D Systems #MAB9589-SP
Anti-CD20 Functional Antibody BPS Bioscience #71209
SK-BR-3 Cells ATCC #HTB-30
Human B Cells WIL2-S ATCC #CRL-8885
96-well tissue culture-treated white clear-bottom assay plate  
One-Step™ Luciferase Assay System BPS Bioscience #60690
Luminometer  
Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Antibody-dependent cell-mediated cytotoxicity (ADCC) is an immune defense mechanism that involves an effector cell lysing a target cell on which antibodies have bound to specific antigens. The typical ADCC involves activation of natural killer (NK) cells by engaged antibodies. NK cells express Fc receptors, mostly FcγRIIIa (CD16a), on their surface. These Fc receptors recognize and bind the Fc portion of an antibody, such as IgG, when the IgG is bound to the surface of a pathogen-infected target cell. Once the Fc receptor binds to the Fc region of IgG, the NK cell releases cytokines such as IFN-γ and cytotoxic molecules that attack the pathogen-infected target cell. Human FcγRIIIa displays a dimorphism at residue 158. F FcγRIIIa V158 encodes a higher affinity receptor variant with a valine at amino acid residue 158, while F158 encodes a lower affinity receptor variant with a phenylalanine at amino acid residue 158.

References

Cao X., et al., 2022. Science Advances 8 (11): DOI: 10.1126/sciadv.abl9171