Activated Protein C (PROC), His-tag

Catalog #: 70019
Size: 20 µg
$415
Species: Human
Host Species: HEK293 cells
Genbank #: NM_000312
Tag(s): C-terminal His-tag
a.a: 1–461 (end)
Description:
Human activated protein C, also known as PROC, APC, or active coagulation factor XIV, (GenBank Accession No. NM_000312), a.a. 1 – 461 (end) with C-terminal His-tag, expressed in an HEK293 cell expression system. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions.
UniProt
P04070
Synonym(s):
PROC, APC, activated protein C, active coagulation factor XIV
Specific Activity:
≥ 95 pmol/min/µg
Assay Conditions:
50 mM Tris-HCl, pH 8.5, 150 mM NaCl, 10 mM CaCl2, 0.01% Brij-35, 100 µM substrate, and various amounts of Activated Protein C. Incubate for 10 minutes at room temperature. Fluorescence intensity is measured at exc380/em460.
Formulation:
50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, and 250 mM imidazole
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.
Reference(s):
1. Wagener, G., et al., Transplant. Proc. 2012,44(5):1336-1340.
2. Luo, L., et al., Mediators Inflamm. 2012,2012:485279.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Protease
Product Type:
Recombinant Protein
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