Activated DNA
Activated DNA for PARP assay, for use with BPS Bioscience's assay kits for PARPs 1, 2, and 3. PARP1 Chemiluminescent Assay Kit, #80551; PARP2 Chemiluminescent Assay Kit, #80552; PARP3 Chemiluminescent Assay Kit, #80553.
This product has been cited 3 times.
PARP is found in the cell’s nucleus. The main role is to detect and signal single-strand DNA breaks (SSB) to the enzymatic machinery involved in the SSB repair. PARP activation is an immediate cellular response to metabolic, chemical, or radiation-induced DNA SSB damage. Once PARP detects a SSB, it binds to the DNA, and, after a structural change, begins the synthesis of a poly (ADP-ribose) chain (PAR) as a signal for the other DNA-repairing enzymes such as DNA ligase III (LigIII), DNA polymerase beta (POLB), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). Both PARP-1 and PARP-2 are activated by DNA single-strand breaks.
1. Dantzer, F., et al. Methods Enzymol. 2006;409:493-510.\