Activated DNA

Catalog #
80605
$125 *
Size: 500 µl
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Description

Activated DNA for PARP assay, for use with BPS Bioscience's assay kits for PARPs 1, 2, and 3. PARP1 Chemiluminescent Assay Kit, #80551; PARP2 Chemiluminescent Assay Kit, #80552; PARP3 Chemiluminescent Assay Kit, #80553.

This product has been cited 3 times.

Synonyms
DNA, PARP substrate
Product Info
Storage and Usage
Citations3
Supplied As
Aqueous buffer solution.
Format
Aqueous buffer solution
Background

PARP is found in the cell’s nucleus. The main role is to detect and signal single-strand DNA breaks (SSB) to the enzymatic machinery involved in the SSB repair. PARP activation is an immediate cellular response to metabolic, chemical, or radiation-induced DNA SSB damage. Once PARP detects a SSB, it binds to the DNA, and, after a structural change, begins the synthesis of a poly (ADP-ribose) chain (PAR) as a signal for the other DNA-repairing enzymes such as DNA ligase III (LigIII), DNA polymerase beta (POLB), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). Both PARP-1 and PARP-2 are activated by DNA single-strand breaks.

References

1. Dantzer, F., et al. Methods Enzymol. 2006;409:493-510.\