Activated DNA (5x)

Catalog #: 80605
Size: 500 µL
PARP is found in the cell’s nucleus. The main role is to detect and signal single-strand DNA breaks (SSB) to the enzymatic machinery involved in the SSB repair. PARP activation is an immediate cellular response to metabolic, chemical, or radiation-induced DNA SSB damage. Once PARP detects a SSB, it binds to the DNA, and, after a structural change, begins the synthesis of a poly (ADP-ribose) chain (PAR) as a signal for the other DNA-repairing enzymes such as DNA ligase III (LigIII), DNA polymerase beta (pol?), and scaffolding proteins such as X-ray cross-complementing gene 1 (XRCC1). After repairing, the PAR chains are degraded via Poly(ADP-ribose) glycohydrolase (PARG). Both PARP-1 and PARP-2 are activated by DNA single-strand breaks.
Activated DNA (5x) for PARP assay, for use with BPS's assay kits for PARPs 1, 2, 3, and 6. PARP1 Chemiluminescent Assay Kit, #80551 ; PARP2 Chemiluminescent Assay Kit, #80552; PARP3 Chemiluminescent Assay Kit, #80553; PARP6 Chemiluminescent Assay Kit, #80556; PARP2 Homogeneous Assay Kit, #80702
DNA, PARP substrate
Supplied As:
Aqueous buffer solution
Aqueous buffer solution
Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed

Suitable for use with BPS's assay kits to screen for inhibitors of PARPs 1, 2, 3, and 6, or for activating PARP-1 or PARP-2 enzymes in vitro.
1. Dantzer, F., et al. Methods Enzymol. 2006;409:493-510.
Biotinylated NAD can be used for in situ labeling to study the cellular localization and tissue distribution of ADP-ribosylated proteins.
Avoid freeze/thaw cycles
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