ACK1, GST-tag

Catalog #: 40400
Size: 10 µg
$345
Species: Human
Host Species: Sf9 insect cells
MW: 68 kDa
Genbank #: NM_005781
Tag(s): N-terminal GST-tag
a.a: 110–476(end)
Description:
Human Ack1 (GenBank Accession No. NM_005781), a.a. 110-476(end) with N-terminal GST-tag, MW=68 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Synonym(s):
ACK1, activated Cdc42-associated kinase 1
Assay Conditions:
Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 minutes. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Eley, L. et al., Biochem. Biophys. Res. Commun. 371 (4), 877-882 (2008).
2. Grovdal, L.M. et al., Exp. Cell Res. 314 (6), 1292-1300 (2008).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Cytoplasmic Tyr
Product Type:
Recombinant Protein
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Product Datasheets

40400_Lot110420-20.pdf