ACC1, FLAG-His-tags Recombinant

Catalog #

50202

$610 *

Size: 10 µg

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Description

Recombinant human ACC1 (acetyl-CoA carboxylase alpha), full length, encompassing amino acids 1-2383(end). This construct contains a C-terminal Flag-tag followed by a His-tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

acetyl-Coenzyme A carboxylase alpha transcript variant 1, ACC-1, ACC 1, ACC -1 acetyl-coa carboxylase acetyl coa acetyl coenzyme a

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Acetyl-Coenzyme A Carboxylase 1 (ACC1) inhibition curve using a luminescent assay (#79315). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

ACC1 (1-2383(end)-FLAG-His)

Host Species/Expression System

Sf9 insect cells

Purity

≥74%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 100 µg/ml FLAG peptide

271 kDa

Amino Acids

1–2383(end)

Specific Activity

≥58 pmol/min/µg.

Genbank #

NM_198834

UniProt #

Q13085

Tag(s)

C-terminal FLAG-His-tags

References

1. Sinilnikova, O.M. et al., Cancer Epidemiol. Biomarkers Prev. 16 (3), 409-415 (2007)
2. Olsen, J.V. et al., Cell 127 (3), 635-648 (2006)

Application Reference(s):
A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was done according to the Acetyl-Coenzyme A Carboxylase 1 (ACC1) Assay Kit (BPS Bioscience #79315) with various amounts of ACC1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes-Carboxylase

Regulatory Status

Research Use Only

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Datasheets/Protocols

50202 Lot 210812

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ACC1, FLAG-His-tags Recombinant

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