Abl Kinase Mutant (T315I), His-tag Recombinant

Catalog #

40415

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1), GenBank Accession No. NM_005157, a.a. 229-512, with Thr-to-Ile mutation on a.a. 315* and N-terminal His-tag, MW = 33 kDa, expressed in a baculovirus-infected Sf9 cell expression system. *This mutation is often cited as T334I in variant B (GenBank Accession No. NM_007313).

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Synonyms

Abl-1 T315I, Abl-1 T334I, ABL1, Abelson murine leukemia viral oncogene homolog 1, JTK7

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥86%

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% Glycerol and 3 mM DTT

Concentration

2.91 mg/ml

35 kDa

Amino Acids

229–512

Specific Activity

2500 pmol/min/µg

Genbank #

NM_005157

UniProt #

P00519-1

Tag(s)

N-terminal His-tag

References

1. Cao, C., et al. 2003. J. Biol. Chem. 278(15): 12961–7.
2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3): 356–364.

Storage/Stability

At least 6 months at -80°C. Avoid freeze/thaw cycles.

Assay Conditions

ABL (T315I) activity was confirmed using the ABL (T315I) Assay Kit (BPS Bioscience,
#79576) and following the protocol provided with the kit

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Notes

This protein was previously named as Abl Kinase Mutant (T334I), His-tag. The protein has not changed; however, the standard nomenclature for numbering this protein has been revised, based on PDB Accession number 2V7A_A.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only