Abl Kinase Mutant (T334I), His-tag

Catalog #: 40415
Size: 10 µg
$345
Species: Human
Host Species: Sf9 insect cells
MW: 33 kDa
Genbank #: NM_00731
Tag(s): N-terminal His-tag
a.a: 229–512
Description:
Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1), GenBank Accession No. NM_005157, a.a. 229-512, with Thr-to-Ile mutation on a.a. 315* and N-terminal His-tag, MW = 33 kDa, expressed in a baculovirus-infected Sf9 cell expression system. *This mutation is often cited as T344I in variant B (GenBank Accession No. NM_007313).
UniProt
P00519
Synonym(s):
Abl-1(T315I), Abl-1(T344I), ABL1, Abelson murine leukemia viral oncogene homolog 1, JTK7
Specific Activity:
222 pmoles/min/μg.
Assay Conditions:
Activity was determined using a 100% phosphorylated Tyr02 peptide and a Z-lyte kinase assay (Invitrogen). Reaction was incubated at 30°C for 1 hour.
Formulation:
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Cao, C., et al. 2003. J. Biol. Chem. 278(15): 12961–7.
2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3): 356–364.
Notes:
This mutation is also cited as T344I in variant B (GenBank Accession No. NM_007313).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Receptor Tyrosine
Product Type:
Recombinant Protein
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40415_Lot80828.pdf