A20, His tag, FLAG-tag (Sf9-derived) Recombinant

Catalog #

80408

$440 *

Size: 50 µg

Qty

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Description

Recombinant human A20, also known as tumor necrosis factor-induced protein 3 (TNFAIP3), full length, encompassing amino acids 2-790(end). This construct contains an N-terminal Flag-tag and a C-terminal His-tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C, A20

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥79%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween- 20, 3 mM DTT, and 20% glycerol

91 kDa

Amino Acids

2–790(end)

Specific Activity

0.77 pmol/min/µg

Genbank #

NM_006290

UniProt #

P21580

Tag(s)

N-terminal FLAG-tag, C-terminal His-tag

References

1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.
2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.
3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and various amount of A20 in a volume of 50 μL. Reaction was incubated at 37°C for 30 min and fluorescent signal was measured at excitation of 360 nm, and emission at 460 nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitins: Deubiquitinase (DUB)

Regulatory Status

Research Use Only

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Datasheets/Protocols

80408_Lot_171108

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A20, His tag, FLAG-tag (Sf9-derived) Recombinant

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