1,10-Phenanthroline, Monohydrate

Catalog #: 27713
Size: 0.5 ml
$200
MW: 198.2 Da
CAS Registry #: 5144-89-8
Formula: C12H8N2 · H2O
Description:
1,10-phenanthroline (o-phenanthroline) is a chelator with high affinity for divalent metal ions. When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn2+ ion. As such, o-phenanthroline is widely used when preparing cell lysates for enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family.
Synonym(s):
o–phenanthroline
Purity:
≥99%
Supplied As:
0.5M solution in anhydrous DMSO (100X)
Biological Activity:
Metalloprotease inhibitor; chelates iron, zinc and other divalent metals. Effective concentration 1-10 mM.
Format:
DMSO solution
Application(s):
Useful for inhibition of JAMM-family isopeptidases. Especially valuable for protecting K63-linked polyubiquitin chains in cell lysates.
Reference(s):
1. Cooper, E. M., Cutcliffe, C., Kristiansen, T. Z., Pandey, A., Pickart, C. M., and Cohen, R. E. (2009) K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1. EMBO J. 28:621-631.
2. Sigman, D.S., et al., Nuclease activity of 1,10-phenanthroline-copper in study of protein DNA interactions. Methods Enzymol., 208:414-433 (1991).
Warning(s):
Protect from moisture. Avoid freeze/thaw cycles.
Scientific Category:
Ubiquitin Enzymes
Product Type:
Inhibitors/Activators
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